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| Registros recuperados: 237 | |
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| Rüttler,M.E.; Yanzón,C.S.; Cuitiño,M.J.; Renna,N.F.; Pizarro,M.A.; Ortiz,A.M.. |
| Enteroaggregative Escherichia coli (EAEC) has been implicated in sporadic diarrhea in children and adults and has been identified as the cause of several outbreaks worldwide. The HEp-2 test remains the gold standard for identification of this pathotype. A 60-65 MDa plasmid encodes the aggregative adherence fimbriae (AAF/I and AAF/II), a transcriptional activator ( aggR gene), the enteroaggregative heat-stable enterotoxin EAST1 ( astA gene) and a cytotoxin (Pet). The standard assay for EAEC is performed only in research laboratories, because it is expensive, labor intensive and time-consuming. The Polymerase Chain Reaction (PCR) offers the possibility of rapid diagnosis. In the current study, a multiplex PCR assay which checks aggR and astA genes was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diarrhea; Escherichia coli; Cell culture; PCR (Polymerase Chain Reaction). |
| Ano: 2006 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000200006 |
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| Beloti,Vanerli; Souza,Juliana Aparecida de; Barros,Márcia de Aguiar Ferreira; Nero,Luís Augusto; Mattos,Marcos Rodrigues de; Gusmão,Viviane Vieira; Moraes,Luciane Bilia de. |
| Petrifilm EC and HS were compared to the MPN method to determine their efficacy to enumerate total coliforms and E. coli in 145 samples of water (76 water in natura, 69 drinking water). For water in natura, Petrifilm HS and EC showed good correlation with MPN method. In chlorinated water (< 20 col/100 mL and negatives) the agreement was low. For E.coli enumeration, Petrifilm EC showed a good correlation with MPN method. The results indicated that Petrifilm EC and HS are accurate to enumerate coliforms and E. coli in water when the expected counts are higher than 20/100 mL. |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Water; Coliforms; Escherichia coli; Petrifilm. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400002 |
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| Pereira,Andrea S.; Andrade,Soraya S.; Monteiro,Jussimara; Sader,Helio S.; Pignatari,Antonio C.C.; Gales,Ana C.. |
| Increasing quinolone resistance has been reported worldwide, mainly among clinical isolates of Escherichia coli. The objectives of this study were to determine the susceptibility profile, the genetic relatedness, and the prevalence of the qnr gene among ciprofloxacin-resistant Escherichia coli isolated from distinct Brazilian hospitals. A total of 144 ciprofloxacin-resistant Escherichia coli were isolated from 17 Brazilian hospitals between January/2002 and June/2003. The antimicrobial susceptibility testing was performed by microdilution according to NCCLS. The presence of the qnr gene was initially screened by colony blotting, and then confirmed by PCR followed by DNA sequencing. Ninety-five urinary ciprofloxacin-resistant Escherichia coli were further... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Quinolone resistance; Escherichia coli; PFGE; Qnr. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000100011 |
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| MENDES,Luiz Cláudio Nogueira; MARQUES,Luiz Carlos; SCHOCKEN-ITURRINO,Ruben Pablo; ÁVILA,Fernando Antônio de; MALHEIROS,Euclides Braga. |
| Sixteen adult horses were randomly divided into 4 equal groups (GI, GII, GIII and GIV) of 4 animals and each group was injected intraperitoneally with one of the following suspension: Group I, 100 x 10(7) colony-forming units (CFU) of E. coli diluted in 500 ml of 0.9% saline; Group II, 100 x 10(7) CFU of Bacteroides fragilis in 500 ml of 0.9% saline; Group III, 100 x 10(7) CFU of E. coli in combination with 100 x 10(7) CFU of B. fragilis in 500 ml of 0.9% saline; Group IV, 500 ml of 0.9% saline. Leukopenia appeared in all animals inoculated with bacteria within the first six hours of the experiment. After this period, leukocytosis was observed in some inoculated horses. Horses inoculated with pure cultures of either E. coli or B. fragilis demonstrated mild... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Peritonitis; Horses; Escherichia coli; Bacteroides fragilis. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000200011 |
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| Liu,Zixuan; Zhang,Jizhou; Fan,Hongqiong; Yin,Ruofeng; Zheng,Zhong; Xu,Qian; Liu,Qing; He,Haiting; Peng,Xiaofan; Wang,XinXin; Li,Xiaokun; Xiao,Yechen. |
| Background Overexpression or mutated activation of Fibroblast growth factor receptor 3 (FGFR3) is involved in the pathogenesis of many tumors. More and more studies focus on the potential usage of therapeutic antibodies against FGFR3. Results In this study, a novel single-chain Fv (ScFv) against FGFR3 was prepared and characterized. To achieve the soluble expression, ScFv was fused with Sumo (Small ubiquitin-related modifier) by polymerase chain reaction (PCR), and cloned into pET-20b. The recombinant bacteria were induced by 0.5 mM Isopropyl-ß-d-thiogalactopyranoside (IPTG) for 16 h at 20°C, and the supernatant liquid of Sumo-ScFv was harvested and purified by Ni-NTA chromatography. After being cleaved by the Sumo protease, the recombinant ScFv was... |
| Tipo: Journal article |
Palavras-chave: Escherichia coli; Fibroblast growth factor receptor 3; Single-chain Fv antibody; Soluble expression; Sumo tag. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000400008 |
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| Liberatore,Ana Maria Alvim; Tomita,Sandra Kimie; Vieira,Mônica Aparecida Midolli; Toti Jr.,Cyro; Heckmaier,João; Gomes,Tânia Aparecida Tardelli. |
| The virulence attributes of 56 Escherichia coli strains isolated from sick horses (secretions of uterine cervices; gastrointestinal and lung fragments of necropsy; diarrheic feces, and tracheal washings) was examined by determining their adherence pattern to HeLa cells and searching for the presence of virulence genes of the various E. coli pathotypes. Two non-adherent strains presented astA, which encodes the enteroaggregative E. coli heat-stable toxin. Twenty-seven strains (48.2%) adhered to HeLa cells, 21 (77.8%) of which presented the aggregative adherence pattern (AA) that characterize the Enteroaggregative E. coli pathotype (EAEC). Nine of the strains presenting AA were isolated from secretions of uterine cervix, including one carrying virulence... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Horse; Escherichia coli; Aggregative adherence; Virulence. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100003 |
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| Chah,K.F.; Oboegbulem,S.I.. |
| One hundred and seventy-two ampicillin-resistant E. coli strains isolated from commercial chickens in Enugu State, Nigeria, were screened for beta-lactamase production using the broth method with nitrocefin® as the chromogenic cephalosporin to detect enzyme production. Beta-lactamase producing strains were further examined for extended-spectrum beta-lactamase (ESBL) production using the Oxoid combination discs method. One hundred and seventy (98.8%) of the 172 ampicillin-resistant E. coli strains produced beta-lactamase enzyme. Sixteen (9.4%) beta-lactamase producers were phenotypically confirmed to produce ESBLs. Six of the ESBL producing strains were only detected with ceftazidime versus ceftazidime/clavulanate combination while ten of the ESBL producers... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ampicillin-resistant; Escherichia coli; Beta-lactamase; Extended-spectrum. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400018 |
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| Segura, Audrey; Auffret, Pauline; Bibbal, Delphine; Bertoni, Marine; Durand, Alexandra; Jubelin, Gregory; Kerouredan, Monique; Brugere, Hubert; Bertin, Yolande; Forano, Evelyne. |
| Healthy cattle are the primary reservoir for O157:H7 Shiga toxin-producing E coil responsible for human food-borne infections. Because farm environment acts as a source of cattle contamination, it is important to better understand the factors controlling the persistence of E. coil O157:H7 outside the bovine gut. The E. coil O157:H7 strain MC2, identified as a persistent strain in French farms, possessed the characteristics required to cause human infections and genetic markers associated with clinical O157:H7 isolates. Therefore, the capacity of E. coil MC2 to survive during its transit through the bovine gastro-intestinal tract (GIT) and to respond to stresses potentially encountered in extra intestinal environments was analyzed. E. coil MC2 survived in... |
| Tipo: Text |
Palavras-chave: Escherichia coli; STEC; EHEC; O157:H7; Biofilms; Stress response; Persistence. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00434/54588/55972.pdf |
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| Ribeiro,M.G.; Costa,E.O.; Leite,D.S.; Langoni,H.; Garino Júnior,F.; Victória,C.; Listoni,F.J.P.. |
| Avaliou-se a ocorrência de fatores de virulência e do sorotipo O157:H7 em 120 linhagens de Escherichia coli, isoladas de 80 casos de mastite clínica bovina e 40 de mastite subclínica. Verificou-se alfa-hemolisina em oito (6,7%) linhagens, isoladas de cinco casos de mastite clínica e três de mastite subclínica e em nenhuma das estirpes detectou-se enteroemolisina. A presença de sideróforos foi encontrada em 11 (9,2%) linhagens, sete de mastite clínica e quatro de subclínica. Em duas (1,7%) estirpes isoladas de mastite subclínica, identificou-se enterotoxina STa. Observou-se efeito citopático em células vero compatível com a produção de verotoxina-VT em cinco (4,2%) linhagens, duas de mastite clínica e três subclínicas. Em uma (0,8%) linhagem isolada de... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovino; Mastite; Escherichia coli; Virulência; Toxina; Sorotipo O157:H7. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000500004 |
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| Kindlein,Giselle; Ribeiro,Andréa Machado Leal; Canal,Cláudio Wageck; Vieira,Maitê de Moraes. |
| The effects of vitamin E and selenium (Se) supplementation on the immunity of hens vaccinated against a mixture of six swine-pathogenic Escherichia coli (EC) and avian encephalomyelitis virus (AEV) were studied. Antibody production (AbP) was evaluated in ninety 49 to 57-week-old H&N Nick Chick hens fed diets containing 14IU Vitamin E kg-1 (basal diet), 27, 59, 111, or 111IU vitamin E kg-1 + 0.56ppm Se supplementation. At 51 wks of age, half of the hens were vaccinated against EC, and all birds were vaccinated against AEV. At 53-weeks of age, the birds received a second dose of EC vaccine. Blood samples were collected weekly and serum was analyzed by ELISA for anti-EC IgY and was expressed as optical density (OD). Vaccinated hens had higher serum OD... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Avian encephalomyelitis; Escherichia coli; Immunoglobulin; Selenium; Vitamin E. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000500024 |
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| Lu,Jike; Song,Qi; Ji,Zhenyu; Liu,Xin; Wang,Ting; Kang,Qiaozhen. |
| Background The fermentation conditions of recombinant maltose-binding protein fused to neutrophil-activating protein (rMBP-NAP) of Helicobacter pylori were optimized from Escherichia coli TB1 with varying medium, inoculum age and size, time, inducer, pH and temperature in batch fermentation. Results It was revealed that the optimal conditions for the production of rMBP-NAP in shake flask were as follows: M9 medium (with 3% yeast extract powder added), inoculum age of 19 h, inoculum size of 6%, initial pH of 6.6, temperature of 37°C, and 0.7 mmoL/L IPTG inducted 21 h in a 50 mL/250 mL shake flask. The recombinant protein yield was increased from 59 to 592 mg/L after optimization. Fermentation process conducted in a 10 L fermenter with similar conditions... |
| Tipo: Journal article |
Palavras-chave: Escherichia coli; Fermentation; Optimization; Recombinant protein. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000400004 |
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| Drummond,V.O.; Perecmanis,S.. |
| Um total de 127 cepas de Escherichia coli foi isolado de suínos no Distrito Federal, testado para a presença de genes de enterotoxinas (STa, LT-I, LT-II, Stx1 e Stx2) e para resistência antimicrobiana. Das cepas isoladas, oito (6,3%) possuíam genes para enterotoxinas, sendo quatro (3,2%) positivas somente para LT-I, três (2,4%) somente para STa e uma (0,8%) positiva para STa e LT-I. Nenhuma das cepas isoladas apresentou genes para LT-II, Stx1 ou Stx2. Quanto ao perfil de resistência antimicrobiano, os antibióticos com maiores porcentagens de resistência foram lincomicina (100%), sulfonamidas (74,8%) e tetraciclina (70,1%), enquanto os maiores índices de sensibilidade foram observados na norfloxacina (82,7%), gentamicina (75,6%) e sulfametoxazol +... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Escherichia coli; Suíno; Enterotoxinas; Resistência antimicrobiana. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000400010 |
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| Registros recuperados: 237 | |
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